Efeito do alopurinol e do pós-condicionamento na inibição do traumatismo por reperfusão após isquemia da aorta infrarenal em ratos

Abstract

Ischemia is one of the most common and challenging problems that vascular surgeons are facing and is at the same time paradoxical, therefore, to restore blood flow to a tissue can be triggered local or systemic changes more intense than ischemia per se. These complications are related to release of oxygen free radicals. The aim of this study was to evaluate the effects of allopurinol antioxidant and post ischemic conditioning on the deleterious effects of ischemia followed by reperfusion in a standardized ischemia model in rat members’ flows. This study was approved by CEUA the State University of Ponta Grossa. Therefore, we used 30 Wistar rats, female, approximately 3 months old. The animals were randomly divided into five groups: Group A (SHAM ): Animals that were not subjected to ischemia of the caudal members; Group B: Animals subjected to 2 hours of ischemia and reperfusion only once; Group C: animals were given by gavage at a dose of 100 mg / kg of allopurinol, one hour before surgery, they were then subjected to 2 hours of ischemia and reperfusion only once; Group D: Animals were subjected to 2 hours of ischemia and three reperfusion cycles (two minutes each) interleaved with three ischemia cycles (two minutes each) and Group E: Animals received 100 mg / kg of allopurinol, one hour before the surgical procedure, then subjected to 2 hours of ischemia and reperfusion for three cycles (two minutes each) interleaved with three cycles of ischemia (two minutes each). Three days after Blood samples were collected for biochemical analysis: Urea, Creatinine, Aspartate aminotransferase, Alanine aminotransferase, Creatine kinase, Lactate and measurement of the total antioxidant capacity by the method based on 2.2”- azinobis 3- ethylbenzothiazoline -6- acid sulfonic (ABTS) and collection segments of the small intestine for histological analysis. It was observed protective effect of the use of allopurinol and Post conditioning (PCi) ischemic by measuring creatinine, AST, ALT and lactate, which did not show a significant increase. Regarding the Total Antioxidant Capacity was evident the benefit of PCi, but not of allopurinol. As to the histological analyzes, both methods were effective in minimizing the harmful effects of ischemia and reperfusion process mesenteric. As a result it can be concluded that allopurinol and especially PCI exerted a protective effect, reaching statistical significance.