Avaliação do efeito da terapia fotodinâmica com uso de gel de azul de metileno sobre linhagens de células derivadas de carcinoma espinocelular em boca - estudo in vitro

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dc.contributor.advisor-co1Paludo, Katia Sabrina
dc.contributor.advisor-co1Latteshttps://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4772654D1&tokenCaptchar=03AAYGu2SGigmYr4j9gekUIQKTAT30vseWLXyc28VLZiOxNU1554x7lxDrdRJuDakzYgZQfMFXSyAZvVW9DBxz6oYZzmm6Dm0gMWxHp9A-y7RNuH8RMekPytmIGuFtFEOSYFxcEeDvOCJh8dHsbSlNrcH750CGYV0_GVIXMzDiclNWdAayY-XpRCXNoqZRZry753iV09NAzFOTuzADNK3U2W3I1UJG2WjQhLeOWu1gUKBvTV_vklEasqca-kvMBHnKe7R8pR7LOkymKLF0lACHyalJI0WkCcO20d6rIPnp93tPXko1A4CsNdlzS-iFpkEpNjOCuiUT6PTn0pUzxLSnAvh9Nmc-B_vLMX-Af5LKur9EZ0I-jdD7JX1m9DfMXYjLZ5Pmcjqb9Ciydq0uh8DbaRiY6i7GokxpZeoZ6xTKF7GmO_5RZHmxpx3aTblvoxL6g-fXr8HwtGOBwTSRz0wG7lTSD4omdUfXxmhIa9Oodu0iGWzKYgNnK-th8VTLfDfQ24CNQQrKl13MCzeswNTEFsCP_6tlF2Z-0XVwfTwGnybpD_1GMuja8ITGuPI4kNPtWoNuh3OpPTVSCh_gHXlL-b-HaNL79zLgTgpt_BR
dc.contributor.advisor1Campagnoli, Eduardo Bauml
dc.contributor.advisor1Latteshttps://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771395E4&tokenCaptchar=03AAYGu2TPcUEyVJiySN381MNqzPwfPfGFT2kLaJiZ5Or21ofgVLOl-L6TkDLhvcNXe0T8N61NfY2sGoa-5Hs6CoCaD4ojmUXr4gJc_ySgNb1NCY1nI9-mhw6yyeA8qw4122onPiJJCbGmsCwAiC9fSi45wQDU5rbJdBhPRPUupBJZWsfz5MIETDxRnCd_gPZG16UynYzvdAv67Bzwd5lVwF8qrCmv5lLaYDkk8Kx86ayFST2ZDd6igU5uvKSEwOaZlrG8anIX4dcxEwvhuIZCF3rEgHhHtd7ZA1CxgGWdZfV8y6tsGOWjewrQAhEAe7ltmQyb9dVM1PnBUNxnYVHFhPXg6HeoRMe4NMxKYHlcesOHCMAZZe6Q17JhoZTvri8DfxJR4VSxjd5rW81wyaiOubkwEIBSm3I4aHofaGjgWglQRIhsvWHox55nl2VXo774RiFcBxQid5XS-PHtt4AD-IN3xeA3gtiLbmelJ-Ag-MuklP5rUjrveablim-j12ILET4NUc52GUM3dzFShzg4tobLMZbcNcg4FeZZUR2_7x5YTR_JCCVh0pNTTTQbZKodw2l3S_VHAozAlHloJTY0IWYLMLzw5Yeb3gpt_BR
dc.contributor.referee1Lisboa, Alessandro Hyczy
dc.contributor.referee1Latteshttps://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4777259A8&tokenCaptchar=03AAYGu2S0hR_PZnp8k2hs7tNZxd1Zomq5dK-v7kF7_-URcuVJkYqoSx0szYmlcY1vtBLXk0sxOwYv3CipRd0pMVeY7CIDj_k7kwgAx3iTgkveg4VKu7gc7NgKJkAQdgiTWoK0KWnfqOoPIiK8s5CzsO94ELK75lEVWIlJKCjFv1e6MtzXzmKFtiwVvm9GYDJZetjoI0YP-Ln3PxxOeTpMeraLjMNyKrcwuOzvDogVlqDuuBY2f5EhwqSqHhRG4-Qox-_wWu3__LGAW-FiDwQOtDpZmCxgwOeVLQb1Dio_Z4Mc2sMxiYEL0THonPgsG6aYZ0cSeR0bCg01lS3qTq0dsHTA5jizxJJpPDXsDHtSAwmS7OlSwvzfgcV6LrzGETpEdKPrmUgZ3kW7kgKeJ2Nkr_JtKSlsz6mj3RD_wy7nydaULUFqWIPmMDn8IgN6KDZh1_o4wUc_8I3VHSb3CMeu456ShywSOGYYzfdbA994WdmXndGpflBaHM7Ap2MD_giD6zL2iu_mz-_7dmu9akya1Wph6Ar8pbfNMd1_Qp0yWd4KXeeYC9tHyoDA4xdJXAYTRRa4IxQ0ypVfpt_BR
dc.contributor.referee2Scomparin, Dionizia Xavier
dc.contributor.referee2Latteshttps://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705110U7&tokenCaptchar=03AAYGu2T3m1-dOii5WQcfcJTXqw46BnAb5ew5QN0BH4W6sIj0N03hlE9iLSsokEGlk67mNpD4zKDXgLow7Bkzl8l1fWd-mbCGKjQScgW0dBCKsE9pU0IUtpZ0MoAlLALq0snRfc4jlanpF6k5AeiQLgzQLUW0iTn-7KhrzbXEq0kTHq2mWaU2gNf7NTldpdf8u7EXvcWbfJId8nGWBB8WW4uA23RmPA8-Gbt9koxbXrqBNFAl5fRFNlJr6k8y7lBZ_LT8wdWFfnwiYCPaWyHJYVQ-kve3Ay9WxenJjUG0e7SMajhUS7CaCx852Z4M9KyAp1gGBDAGkJ84aAsQXXJ-ygmonT51Wp1KacSYDajxDFQ4hFnetS7Z5JWvbAvrfBStu3ofkK0vRWvWuao04zcsLWHyZ31k0Dai4oxuNhE2jk3FIAyRzLKP07Lb4JiTDIJHPa6k_6BQpg3aG29D650xqaF7o4AShY7PMIUwZ8gpNd9NAAmnhqoGdc2KQVH14ZqZwRLRx4UHjEJqyPO3krbcg5A5-xVzF8n964_uhLfvVxIFUVLsZdupbEduVAkfDcFZtQIHBiOEIH0cpt_BR
dc.creatorVieira, Sabrina Miranda
dc.creator.Latteshttps://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K8679444J1&tokenCaptchar=03AAYGu2RJhL8MQPHGJdXRxjUeMcygyTD1pq89AjBe-g_mfHpTsSj3ve0j5g54rSFnFiXPxFBf1TYpHM0x3_o9bzgYSmeoubLpRyzOAdtCT3G9AqvPVV2rSH2G_kJ_FEGCZbHsqlmml9i9E5cPfCantvwupnehZcWmPiFQ9u4hOGgwpOdfhwjarRk3q_iCd9QOeErdmx-4uUUunhcmkVtMMRwzk0Td4qGBN3dLhNkr6QSN4kXMgiN41n1KCj49AmarVGsWXacUQ9darb-U4h924zOfpniNA582mBStNkWFemlBagvW9x451rh27ptoJtV3_GkICeUFvHJcXfOsyHk0bor_rmMIon6vqC9VxCcbN8gUWLYlGqlcHDf6ZiUyax96rYE4T9WhRHuWD6yzCdwOpQddQGsT-vTBu_OEEX9YMCbHK4-LIe1-_8dgjieh7R2qodFz27hNwyZN3x1rCQ96CTnrM05W9UKR2MpxVsbK6-yb0WlQCCE3rvOB1U4FbBEEvC-AXFZ8HWN8rSpkJigiXoe5pVVvN3zFYNfePcByipQt95rXC9Tjf4WBZrQtJs-1JVCZugHD4_k5p6-8_LQW4m71wgHUJiNymgpt_BR
dc.date.accessioned2023-06-28T18:56:14Z
dc.date.accessioned2026-06-29T13:38:27Z
dc.date.available2023-06-28T00:00:00Z
dc.date.available2023-06-28T18:56:14Z
dc.date.issued2023-02-24
dc.description.abstractPhotodynamic Therapy (PDT) could be an alternative for the treatment of potentially malignant oral disease or for early-stage squamous cell carcinoma. Less costly photosensitizers could be developed and tested, including methylene blue. The aim of this research was to investigate in vitro the effect of photodynamic therapy using methylene blue as a gel in a cell line derived from oral squamous cell carcinoma (SCC- 4). The methylene blue gel was developed at a predetermined concentration and pH of 6. After preparation of the base gel, incorporation and cytotoxicity tests of the gel were performed with methylene blue at concentrations ranging from 25μm to 400μm. Cell viability was determined by experiments with dimethyltiazoldiphenyltetrazolium (MTT) to verify the efficacy of the gel. Based on the data obtained, we conclude that PDT with AM gel causes a decrease in cell viability of SCC-4 cells after 30 minutes of exposure, but without cytotoxicity.pt_BR
dc.description.resumoA Terapia Fotodinâmica (PDT) pode ser uma alternativa para o tratamento das desordens orais potencialmente malignas ou nos casos de carcinoma espinocelular em estágios iniciais. Os fotossensibilizadores com menor custo poderiam ser desenvolvidos e testados; dentre eles, o Azul de Metileno. O objetivo da pesquisa foi avaliar in vitro a ação da Terapia Fotodinâmica utilizando o Azul de Metileno na forma de gel, em linhagem de célula derivada de carcinoma espinocelular de boca (SCC-4). O gel de Azul de Metileno foi desenvolvido em concentração preestabelecida e com pH 6. Após o preparo do gel base foram realizados testes de incorporação e citotoxicidade do gel com Azul de Metileno em concentrações que variam de 25μm a 400μm. A viabilidade celular foi determinada através de experimentos com dimetiltiazoldifeniltetrazolium (MTT), buscando verificar a eficácia do gel. Com base nos dados obtidos, conclui-se que a PDT com gel de AM induz uma diminuição na viabilidade celular de células SCC-4 passados 30 minutos de exposição, porém sem apresentar citotoxicidade.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.identifier.citationVIEIRA, Sabrina Miranda. Avaliação do efeito da terapia fotodinâmica com uso de gel de azul de metileno sobre linhagens de células derivadas de carcinoma espinocelular em boca - estudo in vitro. 2023. Dissertação (Mestrado em Ciências da Saúde) - Universidade Estadual de Ponta Grossa, Ponta Grossa, 2023.pt_BR
dc.identifier.urihttps://ri.uepg.br/handle/123456789/2063
dc.languageporpt_BR
dc.publisherUniversidade Estadual de Ponta Grossapt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentSetor de Ciências Biológicas e da Saúdept_BR
dc.publisher.initialsUEPGpt_BR
dc.publisher.programPrograma de Pós-Graduação em Ciências da Saúdept_BR
dc.rightsAcesso Abertopt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/
dc.subjectNeoplasias bucaispt_BR
dc.subjectAzul de metilenopt_BR
dc.subjectFotoquimioterapiapt_BR
dc.subjectCarcinoma espinocelular de bocapt_BR
dc.subjectSCC-4pt_BR
dc.subjectOral Cancerpt_BR
dc.subjectSquamous Cell Carcinomapt_BR
dc.subjectMethylene Bluept_BR
dc.subjectPhotochemotherapypt_BR
dc.subjectantineoplastic agentpt_BR
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDEpt_BR
dc.titleAvaliação do efeito da terapia fotodinâmica com uso de gel de azul de metileno sobre linhagens de células derivadas de carcinoma espinocelular em boca - estudo in vitropt_BR
dc.typeDissertaçãopt_BR

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