AVALIAÇÃO DA CITOTOXICIDADE DA FASE VAPOR DO ÓLEO ESSENCIAL DE Jasminum officinale EM CALU-3 (ADENOCARCINOMA DE PULMÃO) E 3T3 (FIBROBLASTOS)

Abstract

Lung cancer is among the most common types among all malignancies, with an increase of 2% per year in its incidence. Chemotherapy is the main treatment recommended, but in addition cause collateral effects and also resistance to multiple drugs can occur, that is, the loss of tumor sensitivity to chemotherapeutic agents. Essential oils (OE) may be an option for this refractoriness of tumors to drugs and also present as an antitumor alternative, especially for the possibility of being administered by inhalation. The objective of this work was to evaluate the cytotoxicity of the vapor phase of jasmine OE in Calu-3 cells (human lung cancer adenocarcinoma) and 3T3 cells (murine fibroblast control line model). The vapor phase of jasmine OE reduces cell viability in a dose-dependent manner, as demonstrated by the MTT and SRB reduction assays. The estimated IC 50 for Calu-3 cells was 1495.57 μg / mL and for 3T3 cells was 3795.05 μg / mL. The possible effects associated with cytotoxicity, (1) cellular morphology, (2) cell cycle phase distribution and (3) P-glycoprotein activity were evaluated. Results indicated that cell death was caused by the late apoptosis and necrosis, reduction in the percentage of cells in the sub-G0 and G2/M cell cycle phases. The vapor phase of the jasmine oil presented an inhibitory effect on the activity of the glycoprotein P in synergy with curcumin. These results suggest the possibility of the use of the OE of jasmine, in its vapor phase in the treatment lung cancer, by inhalation. Further studies are needed to knowledge about essential oils, especially the so-called alternative, against normal and tumor cells of the lung, included the mechanism involved.