Potencial amilásico de fungos filamentosos isolados da polpa de batata-doce (Ipomoea batatas)

Abstract

The sweet potato is a rustic culture, widely adapted and with a high starch content, thus having innumerable aptitudes. For the transformation of sweet potatoes into biofuel, some processes are necessary. The success of ethanol production from sweet potatoes depends on the transformation of starch in fermentable sugars. The most used conversion process is enzymatic hydrolysis, which uses commercially available enzymes. However, this process can be carried out through enzymes secreted by filamentous fungi. In this work, filamentous fungi that naturally colonized sweet potatoes were studied. These were isolated, tested for enzymatic activity and identified by microculture. 51 fungi from the pulp of sweet potatoes Beauregard and BRS-Amélia were isolated, 27 of them showing the ability to hydrolyze starch. Of these, six showed an enzyme index ≥ 2.0 and were identified as Aspergillus sp and Penicilium sp. The crude extract of the genus Aspergillus sp., showed the greatest results in the conversion to glucose. This fact indicates the potential of these fungi in the production of amylase, an important enzyme for the production of ethanol.